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Phospho-CaMKII (Thr286) Recombinant Rabbit mAb (S-1251-278)

Calcium/calmodulin-dependent protein kinase type II subunit alpha,CaM kinase II subunit alpha,CaMK-II subunit alpha,CAMKA,KIAA0968,CAMK2A

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6070
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-CaMKII (Thr286)

  • 分子别名

    Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMKA; KIAA0968; CAMK2A

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Synapse

  • Accession

    Q9UQM7

  • 克隆号

    S-1251-278

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Human brain, mouse brain, rat brain

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Ms, Rt
背景介绍

  • Phospho-CaMKII (Thr286) refers to the phosphorylated form of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) at threonine residue 286. CaMKII is a multifunctional serine/threonine protein kinase that plays a crucial role in various cellular processes, particularly in the heart and brain. When phosphorylated at Thr286, CaMKII undergoes autophosphorylation, which enhances its activity and allows it to function in a Ca2+-independent manner. This phosphorylated state is essential for the regulation of cardiac myocyte contraction, synaptic plasticity, and memory formation. Abnormal phosphorylation of CaMKII at Thr286 has been implicated in several pathological conditions, including heart failure and arrhythmias, making it a potential therapeutic target for cardiovascular diseases.

  • 免疫印迹

    • WB result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
      Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated mouse brain lysate 20 µg
      Lane 2: Lambda phosphatase treated mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 48, 60 kDa

    • WB result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
      Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated rat brain lysate 20 µg
      Lane 2: Lambda phosphatase treated rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 48, 60 kDa

  • 斑点杂交

    • Dot blot result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
      Lane 1: CaMKII (Thr286) phospho peptide
      Lane 2: CaMKII unmodified peptide
      Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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