DNA Ligase IV/LIG4 Recombinant Rabbit mAb (S-2237-64)

DNA ligase 4,DNA ligase IV,Polydeoxyribonucleotide synthase [ATP] 4

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6069

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

DNA Ligase IV/LIG4
分子别名

DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

P49917
克隆号

S-2237-64
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Rt
阳性样本

PC-12, rat testis
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Rt
IHC-P 1:200 Rt

应用	稀释度	推荐种属
WB	1:1000	Rt
IHC-P	1:200	Rt

背景介绍

DNA Ligase IV, also known as LIG4, is a crucial enzyme involved in the non-homologous end joining (NHEJ) pathway, which is essential for repairing DNA double-strand breaks. It plays a vital role in maintaining genomic stability by facilitating the ligation of broken DNA ends. LIG4 forms a complex with other proteins such as XRCC4 and XLF, enhancing its stability and activity. This enzyme is particularly important in the repair of DNA damage caused by ionizing radiation and certain chemotherapeutic agents. Mutations or deficiencies in LIG4 can lead to severe immunodeficiency and developmental disorders due to impaired V(D)J recombination, a process critical for the generation of antigen receptor diversity in the immune system.

免疫印迹
- WB result of DNA Ligase IV/LIG4 Recombinant Rabbit mAb
  Primary antibody: DNA Ligase IV/LIG4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 104 kDa
  Observed MW: 104 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded rat colon. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of DNA Ligase IV/LIG4 in rat tissue.