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宿主来源
Rabbit抗原名称
Progranulin分子别名
Progranulin; PGRN; Acrogranin; Epithelin precursor; Glycoprotein of 88 Kda (GP88; Glycoprotein 88); Granulin precursor; PC cell-derived growth factor (PCDGF); Proepithelin (PEPI); GRN免疫原
Recombinant Protein细胞定位
Secreted, LysosomeAccession
P28799克隆号
S-2202-46抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, A431, HepG2, A549纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
Progranulin is a multifunctional protein that plays crucial roles in various biological processes. It is highly expressed in numerous tissues and is involved in cell growth, differentiation, and survival. Progranulin acts as a growth factor, promoting the proliferation of epithelial cells and fibroblasts. It also has anti-inflammatory properties, modulating immune responses by inhibiting the production of pro-inflammatory cytokines. Additionally, progranulin is implicated in the maintenance of tissue homeostasis and repair, particularly in the brain and nervous system. Mutations in the progranulin gene have been linked to neurodegenerative diseases such as frontotemporal dementia, highlighting its importance in neuronal health.
免疫印迹
WB result of Progranulin Recombinant Rabbit mAb
Primary antibody: Progranulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-Progranulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Progranulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-Progranulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Progranulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Progranulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Progranulin in tumor tissue.
Expression of Progranulin in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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