分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Man6P分子别名
M6P; mannose 6-phosphate; N-glycosylation克隆号
S-2745抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human brain, mouse brain, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:250 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
Mannose 6-phosphate (M6P) is a critical glycosylation marker primarily involved in the Golgi processing of lysosome-targeted proteins. After synthesis in the rough endoplasmic reticulum, the oligosaccharide chains of these proteins undergo phosphorylation to generate the M6P signal. This signal is then recognized and bound by M6P receptors (MPRs) on the Golgi membrane, facilitating vesicular transport to deliver the proteins to lysosomes. This mechanism is essential for the proper sorting of lysosomal enzymes. Dysfunction in this pathway (e.g., defective M6P generation or receptor mutations) can lead to lysosomal storage disorders, such as I-cell disease. The discovery of the M6P pathway has advanced the understanding of intracellular protein sorting and metabolic diseases, and it is also being explored for targeted delivery applications in gene therapy.
免疫印迹
WB result of Man6P Recombinant Rabbit mAb
Primary antibody: Man6P Recombinant Rabbit mAb at 1/250 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrateWB result of Man6P Recombinant Rabbit mAb
Primary antibody: Man6P Recombinant Rabbit mAb at 1/250 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Man6P antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Man6P antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Man6P antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)