Klf4 Recombinant Rabbit mAb (S-2158-54)

Krueppel-like factor 4,Epithelial zinc finger protein EZF,Gut-enriched krueppel-like factor,EZF,GKLF,KLF4

价格￥650.00 （供应商现货： 3-5个工作日）

货号 S0B6066

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Klf4
分子别名

Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor; EZF; GKLF; KLF4
免疫原

Recombinant Protein
细胞定位

Nucleus, Cytoplasm
Accession

O43474
克隆号

S-2158-54
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

HT-29, HCT 116, MCF7, Ramos, HepG2, PC-3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
ICC	1:500	Hu

背景介绍

Klf4, also known as Krüppel-like factor 4, is a transcription factor that plays a crucial role in various biological processes. It is involved in cell differentiation, proliferation, and apoptosis, and is highly expressed in epithelial tissues such as the gastrointestinal tract and skin. Klf4 regulates the expression of numerous genes by binding to specific DNA sequences in their promoters, thereby modulating cellular functions. In the context of stem cell biology, Klf4 is one of the four Yamanaka factors used to reprogram somatic cells into induced pluripotent stem cells (iPSCs), highlighting its significance in cellular reprogramming and regenerative medicine. Additionally, Klf4 has been implicated in maintaining the integrity of the intestinal barrier and has potential implications in cancer development and progression, making it an important target for therapeutic interventions.

免疫印迹
- WB result of Klf4 Recombinant Rabbit mAb
  Primary antibody: Klf4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HT-29 whole cell lysate 20 µg
  Lane 2: HCT 116 whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: Ramos whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Lane 6: PC-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 54 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in F9 cells. Anti- Klf4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).