DHODH Recombinant Rabbit mAb (S-2055-30)

Dihydroorotate dehydrogenase (quinone),mitochondrial,DHOdehase,Dihydroorotate oxidase

Datasheet COA

价格￥650.00 （供应商现货： 3-5个工作日）

货号 S0B6064

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

DHODH
分子别名

Dihydroorotate dehydrogenase (quinone), mitochondrial; DHOdehase; Dihydroorotate oxidase
免疫原

Synthetic Peptide
细胞定位

Mitochondrion
Accession

Q02127
克隆号

S-2055-30
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Rt
阳性样本

MCF7, HepG2, C6, rat heart
预测反应种属
(反应种属缩写表)

Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Rt
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Rt
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

Dihydroorotate dehydrogenase (DHODH) is a crucial enzyme located in the inner mitochondrial membrane, playing a vital role in the de novo pyrimidine biosynthesis pathway. It catalyzes the conversion of dihydroorotate to orotate, which is the fourth and only redox step in this pathway. This process is essential for the production of pyrimidine nucleotides, which are fundamental for DNA and RNA synthesis. DHODH is a flavoprotein that relies on ubiquinone (CoQ) for its function, and its activity is closely linked to the mitochondrial electron transport chain. Additionally, DHODH has garnered attention in cancer research due to its role in tumor cell proliferation and its potential as a target for cancer treatment.

免疫印迹
- WB result of DHODH Recombinant Rabbit mAb
  Primary antibody: DHODH Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 50 kDa
- WB result of DHODH Recombinant Rabbit mAb
  Primary antibody: DHODH Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 50 kDa
  This blot was developed with high sensitivity substrate
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling DHODH antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in MCF7 cells. Anti- DHODH antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).