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Rabbit抗原名称
Caspase-8 (Asp374)分子别名
Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE (FLICE); MCH5; CASP8免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q14790克隆号
S-1530-30抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Caspase-8 (Asp374) is the activated form of human caspase-8 that has been proteolytically processed after Asp-374, yielding the p18 large catalytic subunit. This cleavage occurs within the DISC (death-inducing signaling complex) and represents a key activation step that converts the 55 kDa procaspase-8 zymogen into the mature, fully active heterotetrameric protease capable of triggering apoptosis and modulating necroptosis.
免疫印迹
WB result of Caspase-8 (Asp374) Recombinant Rabbit mAb
Primary antibody: Caspase-8 (Asp374) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 10 μg/ml Cycloheximide for 18 hours, then treated with 20 ng/ml hTNF-α for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 43, 60 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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