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PRDM1/Blimp1 Recombinant Rabbit mAb (S-1972-3)

PR domain zinc finger protein 1,BLIMP-1,Beta-interferon gene positive regulatory domain I-binding factor,PR domain-containing protein 1,Positive regulatory domain I-binding factor 1 (PRDI-BF1,PRDI-binding factor 1)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6058

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

PRDM1/Blimp1
分子别名

PR domain zinc finger protein 1; BLIMP-1; Beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1 (PRDI-BF1; PRDI-binding factor 1)
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

O75626
克隆号

S-1972-3
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

THP-1, HeLa, A431, RAW264.7, rat colon
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:500 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu, Ms

背景介绍

PRDM1, also known as Blimp1, is a crucial transcriptional regulator that plays significant roles in various biological processes. It is a zinc finger protein with a complex structure containing multiple functional domains. In the immune system, PRDM1/Blimp1 is essential for the terminal differentiation of B cells into plasma cells, helping to suppress the expression of genes related to B cell proliferation and maintenance of the B cell phenotype, thereby facilitating the transition to plasma cells which are specialized for antibody production. Additionally, it also has important functions in other cell types and developmental contexts, such as influencing the differentiation of certain T cell subsets and participating in the regulation of some developmental processes in tissues like the skin. Its dysregulation has been implicated in various diseases, including certain types of cancers and autoimmune disorders, making it an interesting target for potential therapeutic interventions.

免疫印迹
- WB result of PRDM1/Blimp1 Recombinant Rabbit mAb
  Primary antibody: PRDM1/Blimp1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: THP-1 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 100 kDa
- WB result of PRDM1/Blimp1 Recombinant Rabbit mAb
  Primary antibody: PRDM1/Blimp1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 100 kDa
- WB result of PRDM1/Blimp1 Recombinant Rabbit mAb
  Primary antibody: PRDM1/Blimp1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat colon lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 100 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti-PRDM1/Blimp1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in Raw264.7 cells. Anti-PRDM1/Blimp1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of PRDM1/Blimp1 in tumor tissue.
- Expression of PRDM1/Blimp1 in human tissue.
- Expression of PRDM1/Blimp1 in mouse & rat tissue.