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Rabbit抗原名称
gamma Tubulin分子别名
Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1 (GCP-1); TUBG; TUBG1免疫原
Recombinant Protein细胞定位
Cytoplasm, CytoskeletonAccession
P23258克隆号
S-2204-13抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
U-2 OS, Jurkat, HeLa, NIH/3T3, rat kidney纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
Gamma Tubulin is a crucial protein involved in microtubule nucleation. It is a member of the tubulin family but has distinct functions compared to alpha and beta tubulin. Gamma Tubulin is a key component of the gamma Tubulin ring complex (γ-TuRC), which is essential for the formation of microtubules. This complex is typically localized at the centrosome, the main microtubule organizing center in animal cells. By binding to other proteins and providing a template for the polymerization of alpha and beta tubulin dimers, gamma Tubulin initiates the growth of microtubules. It plays a vital role in various cellular processes such as mitosis, where it helps in the formation of the mitotic spindle, ensuring proper chromosome segregation. Additionally, gamma Tubulin is also involved in the organization of the cytoskeleton in interphase cells, contributing to cell shape maintenance and intracellular transport. Its proper function is critical for normal cell division and overall cellular architecture.
免疫印迹
WB result of gamma Tubulin Recombinant Rabbit mAb
Primary antibody: gamma Tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 48 kDaWB result of gamma Tubulin Recombinant Rabbit mAb
Primary antibody: gamma Tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 48 kDaWB result of gamma Tubulin Recombinant Rabbit mAb
Primary antibody: gamma Tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 48 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-gamma Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-gamma Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-gamma Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-gamma Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-gamma Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-gamma Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of gamma Tubulin in tumor tissue.
Expression of gamma Tubulin in human tissue.
Expression of gamma Tubulin in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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