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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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SOD2/MnSOD (acetyl K68) Rabbit Polyclonal Antibody

Superoxide dismutase [Mn],mitochondrial

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6056
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SOD2/MnSOD (acetyl K68)

  • 分子别名

    Superoxide dismutase [Mn], mitochondrial

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Mitochondrion

  • Accession

    P04179

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    乙酰化

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Ms, Rt
    IHC-P 1:1000 Hu
背景介绍

  • SOD2 (acetyl K68) refers to the acetylation of lysine 68 (K68) on superoxide dismutase 2 (SOD2), a mitochondrial antioxidant enzyme critical for neutralizing superoxide radicals and protecting cells from oxidative stress. This post-translational modification, mediated by acetyltransferases such as SIRT3 (which can also deacetylate it), enhances SOD2 activity, improving its ability to scavenge reactive oxygen species (ROS) and maintain mitochondrial function. Acetylation at K68 influences SOD2’s structural stability and enzymatic efficiency, playing a key role in aging, metabolic regulation, and diseases like cancer, neurodegeneration, and cardiovascular disorders. Dysregulation of SOD2 (acetyl K68) is linked to increased oxidative damage, making it a potential therapeutic target for conditions involving mitochondrial dysfunction and oxidative stress.

  • 免疫印迹

    • WB result of SOD2/MnSOD (acetyl K68) Rabbit pAb
      Primary antibody: SOD2/MnSOD (acetyl K68) Rabbit pAb at 1/1000 dilution
      Lane 1: mouse heart lysate 20 µg
      Lane 2: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25 kDa
      Observed MW: 25 kDa

    • WB result of SOD2/MnSOD (acetyl K68) Rabbit pAb
      Primary antibody: SOD2/MnSOD (acetyl K68) Rabbit pAb at 1/1000 dilution
      Lane 1: rat kidney lysate 20 µg
      Lane 2: rat liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25 kDa
      Observed MW: 25 kDa

  • 斑点杂交

    • Dot blot result of SOD2/MnSOD (acetyl K68) Rabbit pAb
      Lane 1: SOD2/MnSOD (acetyl K68) peptide
      Lane 2: SOD2/MnSOD (acetyl K68) unmodified peptide
      Primary antibody: SOD2/MnSOD (acetyl K68) Rabbit pAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of SOD2/MnSOD (acetyl K68) in tumor tissue.

    • Expression of SOD2/MnSOD (acetyl K68) in human tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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