产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体
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宿主来源
Rabbit抗原名称
Histone H3 (di methyl K79)分子别名
H3K79me2免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431克隆号
S-861-22抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
甲基化反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, Jurkat, Neuro-2a, C2C12, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms
Histone H3 (di-methyl K79), or H3K79me2, is an epigenetic modification where lysine 79 on the histone H3 protein is dimethylated. This mark is primarily deposited by the methyltransferase DOT1L and plays a crucial role in transcriptional regulation, DNA damage repair, and cell cycle progression. H3K79me2 is associated with active gene transcription, particularly at promoter and enhancer regions, and is implicated in maintaining chromatin structure during development and differentiation. Dysregulation of H3K79 methylation is linked to cancers such as leukemia, where aberrant DOT1L activity drives oncogenic gene expression. Additionally, H3K79me2 serves as a recognition site for chromatin-binding proteins, influencing processes like DNA replication and stem cell maintenance. Its dynamic regulation makes it a potential therapeutic target in epigenetic-based diseases.
免疫印迹
WB result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: Neruo-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
斑点杂交
Dot blot result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Lane 1: H3K79me2 peptide
Lane 2: H3K79me1 peptide
Lane 3: H3K79me3 peptide
Lane 4: H3K79un peptide
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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