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Rabbit抗原名称
SYT2分子别名
Synaptotagmin-2; Synaptotagmin IIImported (SytII)免疫原
Synthetic Peptide细胞定位
Synapse, CytoplasmAccession
Q8N9I0抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
TT, mouse cerebellum, rat cerebellum纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Ms, Rt
Synaptotagmin-2 (SYT2) is a synaptic vesicle membrane protein that functions as a calcium sensor and plays a crucial role in fast Ca²⁺ -dependent neurotransmitter release at the presynaptic nerve terminal. It is widely expressed in the brain and is particularly important at the neuromuscular junction. SYT2 contains two calcium-binding domains, C2A and C2B, with the C2B domain being essential for mediating vesicle fusion and neurotransmitter release. Mutations in the SYT2 gene can lead to various neuromuscular disorders, such as distal motor neuropathy and myasthenic syndrome, which are often associated with presynaptic dysfunction at the neuromuscular junction. Recent studies have also shown that SYT2 can serve as a reliable marker for analyzing the development and plasticity of inhibitory synapses formed by parvalbumin-positive interneurons in the visual cortex.
免疫印迹
WB result of SYT2 Rabbit pAb
Primary antibody: SYT2 Rabbit pAb at 1/1000 dilution
Lane 1: TT whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46-75 kDaWB result of SYT2 Rabbit pAb
Primary antibody: SYT2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46-75 kDaWB result of SYT2 Rabbit pAb
Primary antibody: SYT2 Rabbit pAb at 1/1000 dilution
Lane 1: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46-75 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-SYT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Anti-SYT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-SYT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of mouse & rat in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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