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Rabbit抗原名称
LDHB分子别名
L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit (LDH-H); Renal carcinoma antigen NY-REN-46免疫原
Synthetic Peptide细胞定位
Mitochondrion, CytoplasmAccession
P07195抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, HeLa, Jurkat, mouse heart, rat heart预测反应种属
(反应种属缩写表)Cz, Rb, Du, Ck, Op纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
LDHB protein, encoded by the LDHB gene, is a subunit of the lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with the concomitant interconversion of NADH and NAD+ in a post-glycolysis process. This enzyme exists in five different forms, each composed of four subunits, with LDHB subunits combining with LDHA subunits to form various isozymes. The LDHB gene is located on chromosome 12p12.1 and encodes a protein of 334 amino acids. Mutations in the LDHB gene can lead to lactate dehydrogenase B deficiency, but this condition typically does not cause any physical signs or symptoms.
免疫印迹
WB result of LDHB Rabbit pAb
Primary antibody: LDHB Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDaWB result of LDHB Rabbit pAb
Primary antibody: LDHB Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDaWB result of LDHB Rabbit pAb
Primary antibody: LDHB Rabbit pAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-LDHB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-LDHB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-LDHB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-LDHB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-LDHB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of LDHB in tumor tissue.
Expression of LDHB in human tissue.
Expression of LDHB in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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