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Rabbit抗原名称
CTSK分子别名
Cathepsin K; Cathepsin O; Cathepsin O2; Cathepsin X; CTSO; CTSO2免疫原
Synthetic Peptide细胞定位
Secreted, Lysosome, Apical cell membraneAccession
P43235克隆号
S-1370-278抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
U-87 MG, SK-MEL-28, Jurkat, RAW264.7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
Cathepsin K (CTSK) is a lysosomal cysteine protease predominantly expressed in osteoclasts and belongs to the peptidase C1 protein family. It plays a crucial role in bone remodeling and resorption by degrading collagen and other matrix proteins. CTSK is the most active mammalian collagenase and can cleave both the helical and telopeptide regions of type I collagen, which is abundant in bone. Besides its role in bone metabolism, CTSK is also found in various cancers, such as breast cancer and melanoma, where it contributes to tumor invasiveness and metastasis. Mutations in the CTSK gene can lead to pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature.
免疫印迹
WB result of CTSK Recombinant Rabbit mAb
Primary antibody: CTSK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 30 kDaWB result of CTSK Recombinant Rabbit mAb
Primary antibody: CTSK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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