分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
CMTM6分子别名
CKLF-like MARVEL transmembrane domain-containing protein 6; Chemokine-like factor superfamily member 6; CKLFSF6免疫原
Synthetic Peptide细胞定位
Endosome, Cell membraneAccession
Q9NX76克隆号
S-2013-4抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MDA-MB-231, Raji, BxPC-3, HCT 116, HDLM-2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
CMTM6, also known as CKLF-like MARVEL transmembrane domain-containing 6, is a transmembrane protein encoded by the CMTM6 gene, located on chromosome 3. It belongs to the CKLF-like MARVEL transmembrane domain-containing family, which consists of nine members and is involved in various biological processes including autoimmune diseases, cardiovascular diseases, the male reproductive system, hematopoiesis, and cancer development. CMTM6 has been identified as a critical regulator of PD-L1, an immune checkpoint protein. It stabilizes PD-L1 on the cell surface by preventing its lysosome-mediated degradation and ubiquitination, thereby enhancing the ability of tumor cells to evade immune surveillance. High expression of CMTM6 is associated with poor prognosis in several types of cancer, such as glioma, hepatocellular carcinoma, and triple-negative breast cancer.
免疫印迹
WB result of CMTM6 Recombinant Rabbit mAb
Primary antibody: CMTM6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled MDA-MB-231 whole cell lysate 20 µg
Lane 2: unboiled Raji whole cell lysate 20 µg
Lane 3: unboiled BxPC-3 whole cell lysate 20 µg
Lane 4: unboiled HCT 116 whole cell lysate 20 µg
Lane 5: unboiled HDLM-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Low expression: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CMTM6 in tumor tissue.
Expression of CMTM6 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)