CD99 Mouse mAb (S-2121-45)

CD99 antigen,12E7,E2 antigen,Protein MIC2,T-cell surface glycoprotein E2,MIC2,MIC2X,MIC2Y

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6047

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Mouse
抗原名称

CD99
分子别名

CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; MIC2; MIC2X; MIC2Y
免疫原

Recombinant Protein
细胞定位

Membrane
Accession

P14209
克隆号

S-2121-45
抗体类型

Mouse mAb
抗体同种型

IgM
反应种属 ?

Hu, Ms, Rt
阳性样本

U-937, Jurkat, Molt-4, HuT-78, HUVEC, THP-1, SH-SY5Y, mouse spleen, rat spleen, rat thymus
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

CD99, also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein encoded by the CD99 gene. It is widely expressed in various cell types, including hematopoietic cells, endothelial cells, and many types of cancer cells. This protein plays crucial roles in multiple biological processes such as cell adhesion, migration, differentiation, and apoptosis. In the immune system, CD99 is involved in T-cell activation, maturation, and the regulation of leukocyte transmigration. It also has a significant impact on tumor biology, as it is often overexpressed in cancers like Ewing sarcoma and certain leukemias. Engagement of CD99 by specific antibodies can induce caspase-independent cell death in tumor cells, making it a potential therapeutic target.

免疫印迹
- WB result of CD99 Mouse mAb
  Primary antibody: CD99 Mouse mAb at 1/1000 dilution
  Lane 1: U-937 whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: Molt-4 whole cell lysate 20 µg
  Lane 4: HuT-78 whole cell lysate 20 µg
  Lane 5: HUVEC whole cell lysate 20 µg
  Lane 6: THP-1 whole cell lysate 20 µg
  Lane 7: SH-SY5Y whole cell lysate 20 µg
  Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 19 kDa
  Observed MW: 27 kDa
- WB result of CD99 Mouse mAb
  Primary antibody: CD99 Mouse mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 19 kDa
  Observed MW: 27 kDa
- WB result of CD99 Mouse mAb
  Primary antibody: CD99 Mouse mAb at 1/1000 dilution
  Lane 1: rat spleen lysate 20 µg
  Lane 2: rat thymus lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 19 kDa
  Observed MW: 27 kDa
免疫细胞化学
- ICC shows positive staining in Hut78 cells. Anti- CD99 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).