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Actin (plant specific) Rabbit Polyclonal Antibody

Actin-2,ACT2

价格 400.00 供应商现货 : 3-5个工作日
货号 S0B6046
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产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Actin (plant specific)
  • 分子别名

    Actin-2; ACT2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Cytoskeleton
  • Accession

    Q96292
  • 抗体类型

    Polyclonal antibody
  • 抗体同种型

    IgG
  • 反应种属 ?

    Arabidopsis, Rice, Soybean
  • 阳性样本

    Arabidopsis, rice, soybean
  • 纯化方式

    Immunogen Affinity
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Arabidopsis, Rice, Soybean
背景介绍
  • Actin in plants is a highly conserved cytoskeletal protein that plays essential roles in maintaining cell shape, cytoplasmic streaming, cell division, cell expansion, and intracellular transport. Similar to animal cells, plant actin exists in two forms: monomeric G-actin (globular) and polymeric F-actin (filamentous), which dynamically assemble into microfilament networks to regulate cellular processes. Plants possess multiple actin isoforms (e.g., ACT2, ACT7), each exhibiting tissue-specific and developmental stage-dependent expression patterns—for instance, ACT7 is highly expressed in rapidly growing tissues. Actin filaments interact with various binding proteins, including myosin, formin, and profilin, to drive organelle movement (e.g., chloroplast positioning), cytoplasmic streaming, and critical developmental processes such as cell plate formation, pollen tube tip growth, and stomatal movement. Additionally, plant actin undergoes rapid reorganization in response to environmental stimuli (e.g., gravity, mechanical stress, and pathogen attack), enabling adaptation to external challenges. While the actin gene family is evolutionarily conserved, plants have undergone unique gene duplication and functional diversification to support their distinct growth and developmental strategies.

  • 免疫印迹

    • WB result of Actin (plant specific) Rabbit pAb
      Primary antibody: Actin (plant specific) Rabbit pAb at 1/1000 dilution
      Lane 1: arabidopsis whole plant lysate 20 µg
      Lane 2: rice whole plant lysate 20 µg
      Lane 3: soybean whole plant lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

FAQs

斯达特公司的抗体,可以回收利用几次?

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定

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