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Rabbit抗原名称
Actin (plant specific)分子别名
Actin-2; ACT2免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q96292抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Arabidopsis, Rice, Soybean阳性样本
Arabidopsis, rice, soybean纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Arabidopsis, Rice, Soybean
Actin in plants is a highly conserved cytoskeletal protein that plays essential roles in maintaining cell shape, cytoplasmic streaming, cell division, cell expansion, and intracellular transport. Similar to animal cells, plant actin exists in two forms: monomeric G-actin (globular) and polymeric F-actin (filamentous), which dynamically assemble into microfilament networks to regulate cellular processes. Plants possess multiple actin isoforms (e.g., ACT2, ACT7), each exhibiting tissue-specific and developmental stage-dependent expression patterns—for instance, ACT7 is highly expressed in rapidly growing tissues. Actin filaments interact with various binding proteins, including myosin, formin, and profilin, to drive organelle movement (e.g., chloroplast positioning), cytoplasmic streaming, and critical developmental processes such as cell plate formation, pollen tube tip growth, and stomatal movement. Additionally, plant actin undergoes rapid reorganization in response to environmental stimuli (e.g., gravity, mechanical stress, and pathogen attack), enabling adaptation to external challenges. While the actin gene family is evolutionarily conserved, plants have undergone unique gene duplication and functional diversification to support their distinct growth and developmental strategies.
免疫印迹
WB result of Actin (plant specific) Rabbit pAb
Primary antibody: Actin (plant specific) Rabbit pAb at 1/1000 dilution
Lane 1: arabidopsis whole plant lysate 20 µg
Lane 2: rice whole plant lysate 20 µg
Lane 3: soybean whole plant lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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