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Rabbit抗原名称
TRAF2分子别名
TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; RING-type E3 ubiquitin transferase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3; TRAP3免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q12933抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HEK-293, 293T, Molt-4纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu ICFCM 1:500 Hu
TNF receptor-associated factor 2 (TRAF2) is a multifunctional protein that plays a crucial role in mediating signal transduction from members of the TNF receptor superfamily. It is a 56 kDa trimeric protein, consisting of a globular C-terminal TRAF domain and an extended coiled-coil N-terminal region. TRAF2 acts as both an adaptor protein and an E3 ubiquitin ligase, facilitating the activation of downstream signaling pathways such as the JNK/c-Jun and IKK/NF-κB pathways in response to TNFα stimulation. It directly interacts with TNF receptors and forms complexes with other TRAF proteins, such as TRAF1, to mediate anti-apoptotic signals and inhibit caspase activation. Additionally, TRAF2 is involved in various physiological processes, including cell survival, proliferation, and stress responses, and it has been implicated in cancer progression and immune regulation.
免疫印迹
WB result of TRAF2 Rabbit pAb
Primary antibody: TRAF2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 52 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HEK293 (Human embryonic kidney epithelial cell) labelling TRAF2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HEK293 cells. Anti-TRAF2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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