SLC25A51 Recombinant Rabbit mAb (S-2070-74)
Mitochondrial nicotinamide adenine dinucleotide transporter SLC25A51,Mitochondrial NAD(+) transporter SLC25A51,Mitochondrial carrier triple repeat protein 1,Solute carrier family 25 member 51,MCART1
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宿主来源
Rabbit抗原名称
SLC25A51分子别名
Mitochondrial nicotinamide adenine dinucleotide transporter SLC25A51; Mitochondrial NAD(+) transporter SLC25A51; Mitochondrial carrier triple repeat protein 1; Solute carrier family 25 member 51; MCART1免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
Q9H1U9克隆号
S-2070-74抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HT-29, SW480, HCT 116, SW620, LoVo纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu, Ms
SLC25A51, also known as MCART1, is a mammalian mitochondrial NAD+ transporter that plays a crucial role in cellular metabolism and energy production. Located in the inner mitochondrial membrane, it facilitates the transport of NAD+ into mitochondria, which is essential for maintaining mitochondrial NAD+/NADH ratios and supporting mitochondrial respiration. SLC25A51 is involved in various cellular processes, including the regulation of the AKT/mTOR signaling pathway and proline synthesis, which are critical for cell proliferation. Additionally, its expression is regulated by fasting and circadian rhythms, and it is necessary for sustaining the activity of the mitochondrial deacetylase SIRT3. Recent studies have also shown that SLC25A51 is upregulated in various cancers and promotes tumor growth by maintaining mitochondrial acetylation homeostasis.
免疫印迹
WB result of SLC25A51 Recombinant Rabbit mAb
Primary antibody: SLC25A51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: SW480 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: SW620 whole cell lysate 20 µg
Lane 5: LoVo whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-SLC25A51 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-SLC25A51 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SLC25A51 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SLC25A51 in human tissue.
Expression of SLC25A51 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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