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宿主来源
Rabbit抗原名称
PrPC分子别名
Major prion protein; PrP; ASCR; PrP27-30; PrP33-35C; CD230; ALTPRP; PRIP; PRNP免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P04156克隆号
S-2053-29抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-MEL-28预测反应种属
(反应种属缩写表)Gor, Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
The cellular prion protein (PrPC) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein encoded by the PRNP gene and predominantly expressed in neurons, although it is also found in other tissues such as lymphoid organs and the heart. PrPC has a flexible N-terminal tail and a structured C-terminal core, allowing it to interact with a wide range of partners. Its proposed physiological functions include ion balance homeostasis, neuritogenesis, neuronal protection against oxidative stress, and modulation of cell signaling pathways. However, only its role in peripheral nerve myelination homeostasis has been definitively confirmed. Misfolding of PrPC into the pathogenic PrPSc isoform leads to prion diseases, which are characterized by neurodegeneration.
免疫印迹
WB result of PrPC Recombinant Rabbit mAb
Primary antibody: PrPC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 20-37 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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