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Rabbit抗原名称
PDLI7/LMP1分子别名
PDZ and LIM domain protein 7; LIM mineralization protein (LMP); Protein enigma; ENIGMA; PDLIM7免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q9NR12克隆号
S-2150-68抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, SK-OV-3, 293T, HepG2, WI-38纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:50 Hu ICFCM 1:50 Hu
PDLIM7, also known as LMP1 or Enigma, is a protein encoded by the PDLIM7 gene and belongs to the family of PDZ-LIM proteins. It contains a PDZ domain and three LIM domains, which are involved in protein-protein interactions. The PDZ domain binds to actin filaments, while the LIM domains interact with protein kinases. PDLIM7 functions as an intracellular scaffold protein and acts as an adapter, localizing LIM-binding proteins to actin filaments in both skeletal muscle and nonmuscle tissues. It is involved in various biological processes, including the assembly of actin filament-associated complexes essential for signaling, regulation of mesenchymal stem cell osteogenesis, and promotion of cell proliferation. In thyroid cancer, PDLIM7 is overexpressed in a stage-dependent manner and interacts with pathways such as PI3K/AKT, MDM2, and BMP-1, suggesting its potential role in cancer progression.
免疫印迹
WB result of PDLI7/LMP1 Recombinant Rabbit mAb
Primary antibody: PDLI7/LMP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: WI-38 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) labeled with anti-PDLI7/LMP1 antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PDLI7/LMP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-PDLI7/LMP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-PDLI7/LMP1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PDLI7/LMP1 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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