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VGlut1 Recombinant Rabbit mAb (S-1587-19)

Vesicular glutamate transporter 1,VGluT1,Brain-specific Na(+)-dependent inorganic phosphate cotransporter,Solute carrier family 17 member 7,Bnpi,Slc17a7

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6038

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

VGlut1
分子别名

Vesicular glutamate transporter 1; VGluT1; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7; Bnpi; Slc17a7
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Synapse
Accession

Q3TXX4
克隆号

S-1587-19
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

human brain, mouse brain, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:2000 Hu, Ms, Rt
IF 1:500 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt
IF	1:500	Hu, Ms, Rt

背景介绍

Vesicular glutamate transporter 1 (VGLUT1) is a crucial protein encoded by the SLC17A7 gene, predominantly found in neuron-rich regions of the brain. It is a vesicle-bound, sodium-dependent phosphate transporter that plays a vital role in packaging glutamate into synaptic vesicles, which is essential for excitatory neurotransmission. VGLUT1 is primarily expressed in the neocortex, cerebellum, and hippocampus, and is involved in intercortical and corticothalamic projections. Its function relies on a proton gradient across the vesicular membrane, established by a V-type ATPase, allowing it to couple proton movement out of the vesicle with glutamate transport into it. This protein is also implicated in various neurological disorders, including learning and memory disorders, Alzheimer's disease, and pain pathology.

免疫印迹
- WB result of VGlut1 Recombinant Rabbit mAb
  Primary antibody: VGlut1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled mouse liver lysate 20 µg
  Lane 2: unboiled mouse brain lysate 20 µg
  Negative control: unboiled mouse liver lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 62 kDa
  Observed MW: 56 kDa
- WB result of VGlut1 Recombinant Rabbit mAb
  Primary antibody: VGlut1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled rat liver lysate 20 µg
  Lane 2: unboiled rat brain lysate 20 µg
  Negative control: unboiled rat liver lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 62 kDa
  Observed MW: 56 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebellum. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-VGlut1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse cerebellum. Anti-VGlut1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of VGlut1 in human tissue.
- Expression of VGlut1 in mouse & rat tissue.