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Rabbit抗原名称
Collagen I分子别名
Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1免疫原
Synthetic Peptide细胞定位
Secreted, Extracellular space, Extracellular matrixAccession
P02452克隆号
S-1473-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HFF纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IP 1:50 Hu IHC-P 1:250 Hu ICC 1:500 Hu
Collagen I protein is the most abundant collagen in the human body, making up around 90% of the total collagen in vertebrates. It is a major structural component of connective tissues, including skin, tendons, ligaments, bones, and the organic part of teeth. Collagen I is composed of a triple helix structure formed by two α1 chains and one α2 chain encoded by the COL1A1 and COL1A2 genes, respectively. This protein provides significant tensile strength and elasticity to tissues, allowing them to withstand mechanical stress. Its synthesis involves complex processes, including transcription, translation, post-translational modifications, and assembly into collagen fibers. Due to its high biocompatibility and unique physical properties, Collagen I is widely used in pharmaceuticals, regenerative medicine, and clinical applications.
免疫印迹
WB result of Collagen I Recombinant Rabbit mAb
Primary antibody: Collagen I Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HFF whole cell lysate 20 µg
Negative control: HT-29 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 220 kDa
免疫沉淀
Collagen I Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Collagen I in 0.4 mg HFF whole cell lysate.
Western blot was performed on the immunoprecipitate using Collagen I Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HFF whole cell lysate 20 µg (Input)
Lane 2: Collagen I Rabbit mAb IP in HFF whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HFF whole cell lysate
Predicted MW: 139 kDa
Observed MW: 220 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HFF cells (top panel) and negative staining in HT-29 cells (below panel). Anti-Collagen I antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Collagen I in tumor tissue.
Expression of Collagen I in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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