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宿主来源
Rabbit抗原名称
FACL4分子别名
Long-chain-fatty-acid--CoA ligase 4; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 4 (LACS 4); ACS4; LACS4; ACSL4免疫原
Synthetic Peptide细胞定位
Mitochondrion outer membrane, Cell membrane, Endoplasmic reticulum membraneAccession
O60488抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, T-47D, HeLa, 293T纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
FACL4 (Fatty Acid-CoA Ligase 4), also known as ACSL4, is an enzyme that belongs to the long-chain fatty-acid-CoA ligase family, encoded by the ACSL4 gene. This enzyme plays a crucial role in lipid metabolism by converting free long-chain fatty acids into fatty acyl-CoA esters, which are involved in various metabolic pathways including β-oxidation, glycerolipid synthesis, and protein acylation. FACL4 is unique in its preference for arachidonic acid (AA) as a substrate, and it helps regulate the levels of free AA in cells, which is known to induce apoptosis. The protein is highly expressed in tissues such as the brain, testis, ovary, spleen, and adrenal cortex, and its absence may contribute to conditions like intellectual disability or Alport syndrome. Additionally, FACL4 has been implicated in cancer development, with studies showing its upregulation in colon adenocarcinoma and hepatocellular carcinoma, suggesting its potential involvement in tumorigenesis.
免疫印迹
WB result of FACL4 Rabbit pAb
Primary antibody: FACL4 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 79 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FACL4 in tumor tissue.
Expression of FACL4 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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