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Rabbit抗原名称
IgE分子别名
Immunoglobulin heavy constant epsilon; Ig epsilon chain C region; IGHE免疫原
Recombinant Protein细胞定位
Cell membrane, SecretedAccession
P06336克隆号
S-1497-55抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
mouse spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ELISA
WB
稀释度
应用 稀释度 推荐种属 ELISA 1:1000-1:2000 Ms WB 1:1000 Ms
IgE(Immunoglobulin E)is a type of antibody that plays a crucial role in the immune system. It is present in relatively low concentrations in the blood compared to other immunoglobulins like IgG or IgA. IgE is primarily known for its involvement in allergic reactions. When the body encounters an allergen, such as pollen, dust mites, or certain foods, IgE antibodies bind to the allergen and trigger the release of histamine and other inflammatory substances from mast cells and basophils. This leads to the symptoms commonly associated with allergies, including itching, swelling, and sneezing. Additionally, IgE is important in defending against parasitic infections by activating immune responses that help eliminate parasites from the body.
免疫印迹
WB result of IgE Recombinant Rabbit mAb
Primary antibody: IgE Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 85 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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