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VEGFA Recombinant Rabbit mAb (S-5301)

Vascular endothelial growth factor A,long form,L-VEGF,Vascular permeability factor (VPF),VEGF,VEGF-A

价格 ￥600.00 （ 1-2周）
货号 S0B60312
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    VEGFA

  • 分子别名

    Vascular endothelial growth factor A, long form; L-VEGF; Vascular permeability factor (VPF); VEGF; VEGF-A

  • 细胞定位

    Nucleus, Secreted, Cytoplasm

  • Accession

    P15692

  • 克隆号

    S-5301

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

  • 稀释度

    应用 稀释度 推荐种属
    IHC-P 1:100 Hu
    ICC 1:1000-1:2000 Hu
背景介绍

  • Vascular Endothelial Growth Factor A (VEGF-A) is a 45 kDa homodimeric glycoprotein and the founding member of the VEGF family, encoded on chromosome 6p21.3 and expressed as at least seven splice isoforms (e.g., VEGF-A165) that are secreted or extracellular-matrix-bound to initiate angiogenesis and vascular permeability through binding VEGFR-2 on endothelial cells and neuropilin co-receptors, triggering PI3K-Akt, PLCγ-MAPK, and Src signaling cascades that drive endothelial proliferation, migration, and tube formation, while its transcription is up-regulated by hypoxia via HIF-1α and by growth factors such as PDGF and FGF, and its dysregulation underlies tumor neovascularization, diabetic retinopathy, and inflammatory disorders, making VEGF-A a pivotal therapeutic target for anti-angiogenic cancer drugs and anti-VEGF antibodies like bevacizumab.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-VEGFA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-VEGFA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-VEGFA antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of VEGFA in tumor tissues.

    • Expression of VEGFA in human tissues.

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