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宿主来源
Mouse抗原名称
PDHE1 Alpha分子别名
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A; PDHA1细胞定位
MitochondrionAccession
P08559抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, K-562, Jurkat, NIH/3T3, mouse brain, rat brain纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Hu, Ms, Rt IHC-P 1:400-1:1600 Hu, Ms, Rt
PDHE1 Alpha (i.e., pyruvate dehydrogenase E1 subunit alpha, encoded by the PDHA1 gene) is the catalytic core subunit of the first enzyme component (E1) of the pyruvate dehydrogenase complex (PDC), located in the mitochondrial matrix. Through its thiamine pyrophosphate (TPP) cofactor-dependent active site, this protein catalyzes the oxidative decarboxylation of pyruvate to generate a hydroxyethyl-TPP intermediate, which is a critical irreversible step that channels the glycolytic product pyruvate into the tricarboxylic acid (TCA) cycle, thus occupying a central position in aerobic glucose oxidation across the entire cell. The activity of PDHE1 Alpha is finely regulated by a complex phosphorylation/dephosphorylation cycle: specific pyruvate dehydrogenase kinases (PDKs) inactivate it by phosphorylating three serine residues (especially Ser-293), while pyruvate dehydrogenase phosphatases (PDPs) restore its activity through dephosphorylation, allowing flexible regulation of metabolic flux according to substrate and energy supply-demand status. The PDHA1 gene encoding this protein is located on the X chromosome, and its pathogenic mutations are among the most common genetic causes of Leigh syndrome, lactic acidosis, and various early-onset neurodegenerative diseases, with patients often presenting with developmental delay, dystonia, and neurological dysfunction—highlighting the special importance of this protein in maintaining cerebral energy metabolism.
免疫印迹
WB result of PDHE1 Alpha Mouse mAb
Primary antibody: PDHE1 Alpha Mouse mAb at 1/20000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 39 kDaWB result of PDHE1 Alpha Mouse mAb
Primary antibody: PDHE1 Alpha Mouse mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 39 kDa
WB result of PDHE1 Alpha Mouse mAb
Primary antibody: PDHE1 Alpha Mouse mAb at 1/20000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 39 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human heart muscle. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse heart muscle. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat heart muscle. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PDHE1 Alpha in tumor tissues.
Expression of PDHE1 Alpha in human tissues.
Expression of PDHE1 Alpha in mouse & rat tissues.
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