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EGR1 Rabbit Polyclonal Antibody

Early growth response protein 1,EGR-1,AT225,Nerve growth factor-induced protein A (NGFI-A),Transcription factor ETR103,Transcription factor Zif268,Zinc finger protein 225,Zinc finger protein Krox-24,KROX24,ZNF225

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6029
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    EGR1

  • 分子别名

    Early growth response protein 1; EGR-1; AT225; Nerve growth factor-induced protein A (NGFI-A); Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24; KROX24; ZNF225

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P18146

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa treated with TNF-α, NIH/3T3, mouse brain

  • 预测反应种属
    (反应种属缩写表)

    Bv

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:200 Hu, Ms, Rt
    ChIP 1:20-1:50 Hu
背景介绍

  • EGR1 protein, also known as Early Growth Response 1 or NGFI-A, is a mammalian transcription factor encoded by the EGR1 gene. It belongs to the EGR family of Cys2His2-type zinc finger proteins and acts as a nuclear protein regulating transcription. EGR1 plays a crucial role in various biological processes, including cell differentiation, proliferation, and apoptosis. It is involved in the regulation of multiple genes, some of which are tumor suppressors like TGFβ1, p53, and PTEN. However, its role in cancer is complex; while it is overexpressed in some cancers like colorectal and gastric cancer, leading to poor prognosis, it also acts as a tumor suppressor in others, such as rhabdomyosarcoma. Additionally, EGR1 is implicated in neuronal plasticity and cardiovascular diseases.

  • 免疫印迹

    • WB result of EGR1 Rabbit pAb
      Primary antibody: EGR1 Rabbit pAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 20 ng/ml TNF-α for 1 hour whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 58 kDa
      Observed MW: 85 kDa

    • WB result of EGR1 Rabbit pAb
      Primary antibody: EGR1 Rabbit pAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 58 kDa
      Observed MW: 85 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa+hTNF-α (20 ng/ml, 5 min) cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used EGR1 Rabbit Polyclonal Antibody and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
      qPCR showed the enrichment of ATAD2, EGR1 and SAT-α in EGR1 Rabbit
      Polyclonal Antibody-immunoprecipitated sample.

  • 组织表达图谱

    • Expression of EGR1 in tumor tissue

    • Expression of EGR1 in human tissue

    • Expression of EGR1 in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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