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Rabbit抗原名称
DGKA分子别名
Diacylglycerol kinase alpha; DAG kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha (DGK-alpha); DAGK; DAGK1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P23743克隆号
S-1933-121抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Jurkat, HeLa, HEK-293, Molt-4纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:100 Hu
Diacylglycerol kinase alpha (DGKA) is an enzyme encoded by the DGKA gene in humans and belongs to the eukaryotic diacylglycerol kinase family. It is involved in intracellular signaling pathways, acting as a modulator that competes with protein kinase C for the second messenger diacylglycerol. DGKA also plays a crucial role in the resynthesis of phosphatidylinositols and the phosphorylation of diacylglycerol to phosphatidic acid. Additionally, DGKA has been identified as a potential candidate in radiation-induced fibrosis, where its expression can be induced by the transcription factor EGR1, leading to enhanced production of pro-fibrotic ECM proteins such as collagen and fibronectin.
免疫印迹
WB result of DGKA Recombinant Rabbit mAb
Primary antibody: DGKA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 83 kDa
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- DGKA antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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