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Rabbit抗原名称
ALDH2分子别名
Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDM免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P05091克隆号
S-4173-20抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
THP-1, Ramos, SK-BR-3, HepG2, mouse liver, mouse kidney, rat liver, rat kidney纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1: 1000-1:5000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
Aldehyde dehydrogenase 2 (ALDH2) is a crucial mitochondrial enzyme that serves as the primary catalyst for the oxidation of acetaldehyde, a toxic byproduct of ethanol metabolism, into harmless acetate, thereby playing a pivotal role in alcohol detoxification and cellular protection against oxidative stress. Encoded by the ALDH2 gene on chromosome 12, this homotetrameric protein is particularly significant in East Asian populations, where a common genetic variant (Glu504Lys, known as ALDH2*2) results in a dominant-negative loss of enzymatic activity, leading to the characteristic "Asian flush" syndrome characterized by facial flushing, nausea, and tachycardia upon alcohol consumption due to acetaldehyde accumulation. Beyond its central function in alcohol metabolism, ALDH2 is increasingly recognized for its broader cytoprotective roles, including the detoxification of reactive aldehydes generated during lipid peroxidation, which links its activity to the pathogenesis of various diseases such as cardiovascular disorders, neurodegenerative conditions, and cancer, making it a promising therapeutic target for mitigating tissue damage induced by oxidative stress and ischemia-reperfusion injury.
免疫印迹
WB result of ALDH2 Recombinant Rabbit mAb
Primary antibody: ALDH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 55 kDaWB result of ALDH2 Recombinant Rabbit mAb
Primary antibody: ALDH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 53 kDaWB result of ALDH2 Recombinant Rabbit mAb
Primary antibody: ALDH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 54 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALDH2 in tumor tissues.
Expression of ALDH2 in human tissues.
Expression of ALDH2 in mouse & rat tissues.
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