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宿主来源
Rabbit抗原名称
VGLUT2分子别名
Vesicular glutamate transporter 2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6; Dnpi; Vglut2; Slc17a6免疫原
Synthetic Peptide细胞定位
Cell membrane, SynapseAccession
Q8BLE7克隆号
S-4399-14抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse brain, rat brian纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Ms, Rt
VGLUT2 (Vesicular Glutamate Transporter 2), also known as SLC17A6 or DNPI, is a synaptic vesicle protein that mediates the sodium-dependent uptake of glutamate into presynaptic vesicles. As a specific marker for glutamatergic neurons, it plays a crucial role in excitatory neurotransmission and is considered a potential therapeutic target for neurological disorders involving excessive glutamate signaling, such as epilepsy, chronic pain, and neurodegenerative diseases.
免疫印迹
WB result of VGLUT2 Recombinant Rabbit mAb
Primary antibody: VGLUT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65-70 kDa
Observed MW: 65 kDaWB result of VGLUT2 Recombinant Rabbit mAb
Primary antibody: VGLUT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65-70 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse spleen. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VGLUT2 in mouse & rat tissues.
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