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宿主来源
Rabbit抗原名称
TUBA1A分子别名
Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA3; TUBA1A免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q71U36抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
Ramos, HeLa, A431, K562, MCF7, NIH/3T3, mouse brain, PC-12, COS7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:50-1:100 Hu, Ms, Rt
TUBA1A (Tubulin Alpha 1A) is a neuron-specific alpha-tubulin isotype that serves as a fundamental building block of microtubules in the central nervous system. It is highly expressed in the developing brain and is critical for essential processes like neuronal migration, axon guidance, and cortical organization. Clinically, heterozygous missense mutations in the TUBA1A gene are a well-established cause of a spectrum of severe neurodevelopmental disorders known as tubulinopathies, which include lissencephaly, pachygyria, and polymicrogyria.
免疫印迹
WB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: Hela whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-TUBA1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-TUBA1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-TUBA1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TUBA1A in tumor tissues.
Expression of TUBA1A in human tissues.
Expression of TUBA1A in mouse & rat tissues.
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