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TGFBI / BIGH3 Mouse mAb

Transforming growth factor-beta-induced protein ig-h3,Kerato-epithelin,RGD-containing collagen-associated protein (RGD-CAP),BIGH3,TGFBI

价格 ￥600.00 （ 1-2周）
货号 S0B60280
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    TGFBI / BIGH3

  • 分子别名

    Transforming growth factor-beta-induced protein ig-h3; Kerato-epithelin; RGD-containing collagen-associated protein (RGD-CAP); BIGH3; TGFBI

  • 细胞定位

    Secreted

  • Accession

    Q15582

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG2a

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    U-87 MG, mouse liver, rat eye

  • 纯化方式

    Protein A

  • 浓度

    1.3 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:500 Hu
背景介绍

  • TGFBI (Transforming Growth Factor Beta-Induced), also known as βig-H3, is an extracellular matrix protein that plays a critical role in cell adhesion, bone formation, and wound healing. Clinically, it is best known for its association with hereditary corneal dystrophies, where mutations lead to abnormal protein accumulation in the eye. In cancer biology, TGFBI exhibits a context-dependent dual role: it can act as a tumor suppressor in early stages by enhancing chemotherapy sensitivity, but often functions as a tumor promoter in established tumors by facilitating metastasis and cell survival.

  • 免疫印迹

    • WB result of TGFBI / BIGH3 Mouse mAb
      Primary antibody: TGFBI / BIGH3 Mouse mAb at 1/1000 dilution
      Lane 1: U-87 MG whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 75 kDa
      Observed MW: 68 kDa
      This blot was developed with high sensitivity substrate

    • WB result of TGFBI / BIGH3 Mouse mAb
      Primary antibody: TGFBI / BIGH3 Mouse mAb at 1/1000 dilution
      Lane 1: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-Mouse (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 75 kDa
      Observed MW: 55 kDa
      This blot was developed with high sensitivity substrate

    • WB result of TGFBI / BIGH3 Mouse mAb
      Primary antibody: TGFBI / BIGH3 Mouse mAb at 1/1000 dilution
      Lane 1: rat eye lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 75 kDa
      Observed MW: 52 kDa
      This blot was developed with high sensitivity substrate

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-TGFBI / BIGH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TGFBI / BIGH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TGFBI / BIGH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of TGFBI / BIGH3 in tumor tissues.

    • Expression of TGFBI / BIGH3 in human tissues.

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