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Rabbit抗原名称
TROP1/EPCAM分子别名
Epithelial cell adhesion molecule; Ep-CAM; Epithelial glycoprotein 314 (EGP314; mEGP314); Protein 289A; Tumor-associated calcium signal transducer 1; CD326; Tacstd1; Epcam免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q99JW5克隆号
S-1379-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse colon, rat colon纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000-1:2000 Ms, Rt IF 1:500 Ms, Rt
TROP1, also known as EpCAM (Epithelial Cellular Adhesion Molecule) or CD326, is a 40 kDa type I transmembrane glycoprotein that plays crucial roles in various cellular processes. It consists of an extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats, a transmembrane segment, and a cytoplasmic domain. EpCAM is highly conserved between species, with 82% amino acid sequence identity between humans and mice. It is expressed in various tissues during embryonic development and in adult simple, pseudo-stratified, and transitional epithelia, but not in normal squamous epithelia or mesenchymal tissues. EpCAM is upregulated in proliferating epithelial tissues, during liver regeneration, and in many carcinomas, making it a potential target for cancer therapy. Functionally, EpCAM acts as a homophilic Ca²⁺-independent adhesion molecule, forming dimers and tetramers that interact with the actin cytoskeleton and regulate cell adhesion, proliferation, and epithelial-to-mesenchymal transition.
免疫印迹
WB result of TROP1/EPCAM Recombinant Rabbit mAb
Primary antibody: TROP1/EPCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse colon lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 30, 38 kDaWB result of TROP1/EPCAM Recombinant Rabbit mAb
Primary antibody: TROP1/EPCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 32, 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse colon. Anti-TROP1/EPCAM antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-TROP1/EPCAM antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-TROP1/EPCAM antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat skeletal muscle. Anti-TROP1/EPCAM antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse colon. Anti-TROP1/EPCAM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat colon. Anti-TROP1/EPCAM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of TROP1/EPCAM in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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