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宿主来源
Mouse抗原名称
SIRT2分子别名
NAD-dependent protein deacetylase sirtuin-2; NAD-dependent protein deacylase sirtuin-2; NAD-dependent protein defatty-acylase sirtuin-2; Regulatory protein SIR2 homolog 2; SIR2-like protein 2; SIR2L; SIR2L2; SIRT2细胞定位
Cytoskeleton, Nucleus, Cell membrane, CytoplasmAccession
Q8IXJ6抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Ms, Rt阳性样本
HEK293, LNCaP, mouse brain, rat brain纯化方式
Protein G浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000 Hu, Ms, Rt IHC-P 1:50 Hu, Ms, Rt
SIRT2 (Sirtuin 2) is a primarily cytoplasmic, NAD+-dependent protein deacetylase that regulates critical cellular processes such as the cell cycle, metabolism, and autophagy. As a key upstream regulator of the mitotic surveillance protein BubR1, SIRT2 helps maintain genomic stability and prevent aneuploidy, making it closely linked to aging and cancer development. It exhibits context-dependent roles in tumorigenesis (acting as either an oncogene or tumor suppressor) and is currently being investigated as a potential therapeutic target for age-related disorders, including non-small cell lung cancer and neurodegenerative diseases.
免疫印迹
WB result of SIRT2 Mouse mAb
Primary antibody: SIRT2 Mouse mAb at 1/5000 dilution
Lane 1: HEK293 whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40 kDa
This blot was developed with high sensitivity substrateWB result of SIRT2 Mouse mAb
Primary antibody: SIRT2 Mouse mAb at 1/5000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40 kDaWB result of SIRT2 Mouse mAb
Primary antibody: SIRT2 Mouse mAb at 1/5000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SIRT2 in tumor tissues.
Expression of SIRT2 in human tissues.
Expression of SIRT2 in mouse & rat tissues.
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