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Rabbit抗原名称
KLC1分子别名
Kinesin light chain 1; KLC; KNS2; KLC1免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q07866克隆号
S-4556-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
293T, HeLa, U-87 MG, HT-29, COS7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Mk IHC-P 1:500-1:1000 Hu
KLC1 (Kinesin Light Chain 1) is a component of the kinesin-1 motor protein complex that plays a crucial role in microtubule-based intracellular transport. It functions primarily by coupling specific cargo (such as organelles and vesicles) to the kinesin heavy chain and modulating its ATPase activity. Highly abundant in the brain and spine, KLC1 is essential for axo-dendritic transport, and mutations in the KLC1 gene have been linked to neurodevelopmental disorders and movement abnormalities like dystonia.
免疫印迹
WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65-75 kDa
WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65-75 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KLC1 antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KLC1 antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of KLC1 in tumor tissues.
Expression of KLC1 in human tissues.
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