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Mouse抗原名称
GOLGA2/GM130分子别名
Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein (GM130); GM130 autoantigen; Golgin-95; GOLGA2细胞定位
Cytoplasm, Cytoskeleton, Endoplasmic reticulumAccession
Q08379抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, Jurkat, HeLa, K562, C2C12, moues brain, PC-12纯化方式
Protein G浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:2000 Hu, Ms, Rt
GOLGA2 (Golgin subfamily A member 2), commonly known as GM130, is a peripheral membrane protein localized to the cis-Golgi network that serves as a critical structural scaffold for the Golgi apparatus. It maintains the stacked organization of Golgi cisternae and facilitates vesicle tethering and fusion during ER-to-Golgi transport. Beyond its structural role, GOLGA2 is essential for cell division, where it regulates mitotic Golgi disassembly and promotes spindle pole assembly by interacting with microtubule nucleation factors. Clinically, it is a well-established marker for Golgi structure and is implicated in various cellular processes, including protein glycosylation and cell polarization.
免疫印迹
WB result of GOLGA2/GM130 Mouse mAb
Primary antibody: GOLGA2/GM130 Mouse mAb at 1/2000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 130 kDaWB result of GOLGA2/GM130 Mouse mAb
Primary antibody: GOLGA2/GM130 Mouse mAb at 1/2000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 130 kDaWB result of GOLGA2/GM130 Mouse mAb
Primary antibody: GOLGA2/GM130 Mouse mAb at 1/2000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 130 kDa







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