分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
ENO1分子别名
Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; ENO1L1; MBPB1; MPB1; ENO1细胞定位
Nucleus, Cell membrane, CytoplasmAccession
P06733抗体类型
Mouse mAb抗体同种型
IgM反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, HeLa, PC-3, Jurkat, mouse brain, mouse liver, rat brain, rat liver浓度
0.2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000 Hu, Ms, Rt
ENO1 (Alpha-enolase) is a ubiquitous glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Beyond its canonical metabolic role, ENO1 functions as a multifunctional "moonlighting" protein: when expressed on the cell surface, it acts as a plasminogen receptor involved in fibrinolysis, cell migration, and tumor invasion. Clinically, ENO1 is frequently overexpressed in various cancers and serves as a valuable biomarker for tumor progression and autoimmune diseases.
免疫印迹
WB result of ENO1 Mouse mAb
Primary antibody: ENO1 Mouse mAb at 1/5000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDaWB result of ENO1 Mouse mAb
Primary antibody: ENO1 Mouse mAb at 1/5000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDaWB result of ENO1 Mouse mAb
Primary antibody: ENO1 Mouse mAb at 1/5000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
评论(0)