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Rabbit抗原名称
CNBP分子别名
CCHC-type zinc finger nucleic acid binding protein; Cellular nucleic acid-binding protein (CNBP); Zinc finger protein 9; RNF163; ZNF9; CNBP免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulum, Cytoplasm, NucleusAccession
P62633克隆号
S-4288-3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, HepG2, K-562, Jurkat, LNCaP, Neuro-2a, mouse brain, PC-12, rat brain, COS7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu, Ms, Rt
CNBP (Cellular Nucleic Acid-Binding Protein), also known as ZNF9, is a highly conserved, single-stranded nucleic acid-binding protein that functions as a versatile nucleic acid chaperone. It plays a critical role in regulating both transcription and translation by remodeling nucleic acid secondary structures and modulating the activity of trans-acting factors. CNBP is essential for maintaining sustained immune responses (such as prolonged IL-6 expression) and proper embryonic development. Clinically, abnormal expansions of the CCTG repeat in the first intron of the CNBP gene are the direct cause of myotonic dystrophy type 2 (DM2), a multisystem disorder.
免疫印迹
WB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: K-562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
WB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDaWB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDaWB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CNBP in tumor tissues.
Expression of CNBP in human tissues.
Expression of CNBP in mouse & rat tissues.
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