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宿主来源
Rabbit抗原名称
Caspase-12分子别名
Caspase-12; Casp12细胞定位
Cytoplasm, Endoplasmic reticulumAccession
O08736抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse liver, rat liver纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:500 Ms, Rt
Caspase-12 is a cysteine protease specifically localized to the endoplasmic reticulum (ER) that plays a pivotal role in mediating ER stress-induced apoptosis triggered by stimuli such as calcium overload, hypoxia, and glucose deprivation. It is also closely associated with neurodegenerative processes, as it co-localizes with Alzheimer's disease-related proteins and mediates amyloid-beta-induced cytotoxicity. Notably, while Caspase-12 is fully functional in rodents, the human CASP12 gene contains a premature stop codon in most populations, rendering it a pseudogene with no protease activity.
免疫印迹
WB result of Caspase-12 Recombinant Rabbit mAb
Primary antibody: Caspase-12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 53 kDaWB result of Caspase-12 Recombinant Rabbit mAb
Primary antibody: Caspase-12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Caspase-12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Caspase-12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- Caspase-12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Caspase-12 in mouse & rat tissues.
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