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Rabbit抗原名称
Tenascin C分子别名
Tenascin; TN; Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; HXB; TNC免疫原
Synthetic Peptide细胞定位
SecretedAccession
P24821克隆号
S-775-274抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
U-87 MG, mouse brain, normal mouse E14 foetal brain, normal rat E14 foetal brain预测反应种属
(反应种属缩写表)Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:100-1:200 Hu, Ms, Rt ICC 1:500 Hu
Tenascin-C (TNC) is a large, multimodular, extracellular matrix glycoprotein that exists in several molecular forms (180-250 kDa) due to alternative splicing and protein modifications. It is a hexameric protein with each subunit having a molecular weight of around 300 kDa. TNC has a restricted pattern of expression but performs a wide range of functions. It plays crucial roles in cell proliferation, migration, and adhesion modulation, inhibiting cellular adhesion to fibronectin. It is highly expressed during embryonic development, tissue remodeling, wound healing, inflammation, and cancer progression. TNC also regulates immune responses and is involved in both innate and adaptive immunity. Additionally, it has binding partners like fibronectin, collagen, and proteoglycans, and its expression is tightly controlled by various transcription factors and intracellular regulators.
免疫印迹
WB result of Tenascin C Recombinant Rabbit mAb
Primary antibody: Tenascin C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Low expression control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 241 kDa
Observed MW: 190-240 kDaWB result of Tenascin C Recombinant Rabbit mAb
Primary antibody: Tenascin C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: normal mouse E14 foetal brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 241 kDa
Observed MW: 190-300 kDaWB result of Tenascin C Recombinant Rabbit mAb
Primary antibody: Tenascin C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: normal rat E14 foetal brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 241 kDa
Observed MW: 180-300 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Tenascin C antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Tenascin C antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Tenascin C antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Tenascin C antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Tenascin C antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HCT116 cells (top panel) and negative staining in U87-MG cells (below panel). Anti- Tenascin C antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Tenascin C in tumor tissue.
Expression of Tenascin C in human tissue.
Expression of Tenascin C in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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