HSP90 Recombinant Mouse mAb (S-5484)
Heat shock protein HSP 90-alpha,Heat shock 86 kDa (HSP 86,HSP86),Heat shock protein family C member 1,Lipopolysaccharide-associated protein 2 (LAP-2,LPS-associated protein 2),Renal carcinoma antigen NY-REN-38,HSP90A,HSPC1,HSPCA,HSP90AA1
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宿主来源
Mouse抗原名称
HSP90分子别名
Heat shock protein HSP 90-alpha; Heat shock 86 kDa (HSP 86; HSP86); Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2 (LAP-2; LPS-associated protein 2); Renal carcinoma antigen NY-REN-38; HSP90A; HSPC1; HSPCA; HSP90AA1细胞定位
Cytoplasm, NucleusAccession
P07900克隆号
S-5484抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, MCF7, Jurkat, LnCap, HepG2, mouse spleen, mouse brain, rat spleen, rat brain, rat testis, rat colon纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
HSP90 (Heat Shock Protein 90) is a highly conserved and extremely abundant molecular chaperone protein within cells. Existing as a homodimer, it assists numerous "client proteins" in proper folding, maturation, and activity maintenance through ATP-dependent conformational changes. These client proteins are predominantly kinases, hormone receptors, and transcription factors involved in cell proliferation, survival, and signal transduction. Its expression is upregulated when cells respond to external stresses such as heat shock and oxidative stress, where it exerts important cytoprotective functions by stabilizing denatured proteins and preventing their aggregation. Additionally, HSP90 participates in immune regulation—for example, by promoting antigen presentation and TLR signaling pathway activation to enhance innate immune responses, as well as supporting T cell proliferation and differentiation. Since its client protein network includes a large number of oncoproteins (such as HER2, AKT, and BRAF), HSP90 inhibition has become an important strategy in cancer therapy. Furthermore, its dysfunction is closely associated with the accumulation of protein aggregates in neurodegenerative diseases (such as Alzheimer's disease) as well as with fungal infection drug resistance.
免疫印迹
WB result of HSP90 Recombinant Mouse mAb
Primary antibody: HSP90 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: LnCap whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDaWB result of HSP90 Recombinant Mouse mAb
Primary antibody: HSP90 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDaWB result of HSP90 Recombinant Mouse mAb
Primary antibody: HSP90 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Lane 4: rat colon lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of HSP90 in tumor tissues.
Expression of HSP90 in human tissues.
Expression of HSP90 in mouse & rat tissues.
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