分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
Syk分子别名
Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P43405克隆号
S-4400-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Raji, SW620, A20纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
Syk (spleen tyrosine kinase) is a non-receptor tyrosine kinase widely expressed in hematopoietic cells, belonging to the Syk family. Its structural features include two tandem SH2 domains at the N-terminus, which recognize and bind to dual-phosphorylated tyrosine residues within immunoreceptor tyrosine-based activation motifs (ITAMs), thereby mediating its own activation and initiating downstream signaling cascades. It plays a central role in signal transduction through various immune receptors, including the B-cell receptor (BCR), T-cell receptor (TCR), and Fc receptors. By phosphorylating downstream substrates such as BLNK, SLP-76, and VAV, it regulates key immune responses including lymphocyte activation, proliferation, differentiation, and antibody production. Beyond adaptive immunity, Syk also participates in innate immune responses mediated by receptors such as FcγR and Dectin-1 in macrophages, neutrophils, dendritic cells, and mast cells, and plays a role in platelet adhesion and aggregation. Given its central position in immune signaling pathways, Syk dysfunction is associated with various diseases—its hyperactivation can drive allergic diseases, autoimmune disorders (such as rheumatoid arthritis and systemic lupus erythematosus), and B-cell lymphomas. Syk inhibitors (such as fostamatinib) have already been used clinically to treat immune thrombocytopenia and show promising applications in various inflammatory and oncological conditions.
免疫印迹
WB result of Syk Recombinant Rabbit mAb
Primary antibody: Syk Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: SW620 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 68 kDaWB result of Syk Recombinant Rabbit mAb
Primary antibody: Syk Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Syk in tumor tissues.
Expression of Syk in human tissues.
Expression of Syk in mouse & rat tissues.
评论(0)