分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
LIF分子别名
Leukemia inhibitory factor; Differentiation-stimulating factor (D factor); Melanoma-derived LPL inhibitor (MLPLI); INN Name; Emfilermin; HILDA免疫原
Recombinant Protein细胞定位
SecretedAccession
P15018克隆号
S-1045-488抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
LIF (Leukemia Inhibitory Factor) is a multifunctional pleiotropic cytokine belonging to the IL-6 cytokine family. It binds to a composite receptor on the cell surface composed of LIF receptor (LIFR) and gp130 subunits, activating multiple downstream signaling pathways including JAK/STAT3, PI3K/AKT, and MAPK, thereby exerting a wide range of biological functions across various tissues. It is critically important in embryonic development, serving as the classic "stem cell maintenance factor" that maintains mouse embryonic stem cell pluripotency and prevents their differentiation. In the nervous system, it promotes the survival of neural progenitor cells and the differentiation of astrocytes, while also playing a role in promoting repair and regeneration after nerve injury. Furthermore, LIF is deeply involved in immune regulation (such as promoting Th17 cell differentiation), bone metabolism regulation, inflammatory responses, and reproductive physiological processes like endometrial decidualization. Therefore, aberrant LIF expression is closely associated with various diseases, including certain autoimmune disorders, osteoarthritis, neurological diseases, and tumor development and progression. Clinically, recombinant LIF has also been explored for improving embryo implantation to enhance assisted reproductive outcomes.
免疫印迹
WB result of LIF Recombinant Rabbit mAb
Primary antibody: LIF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Cell supernatant from untreated A375 lysate 20 µg
Lane 2: Cell supernatant from A375 cells treated with Oncostatin M (1000 pM, 24 hr) and TNF-α (100 pM, 24 hr) lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 36-45 kDa
评论(0)