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宿主来源
Rabbit抗原名称
Ferritin Heavy Chain分子别名
Ferritin H subunit; HftCleaved into 1 chainFerritin heavy chain; N-terminally processed; Fth; Fth1细胞定位
Cytoplasm, LysosomeAccession
P09528克隆号
S-5419抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
RAW264.7, NIH/3T3, mouse liver, mouse brain, mouse heart, mouse spleen, PC-12, C6, rat live, rat brain, rat kidney, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Ms, Rt
Ferritin Heavy Chain (FTH1) is the catalytic core subunit of the ferritin complex. Through its unique ferroxidase activity, it rapidly oxidizes toxic ferrous iron (Fe²⁺) to non-toxic ferric iron (Fe³⁺) and safely stores it within a spherical protein shell self-assembled from 24 subunits, thereby playing a critical role in maintaining cellular iron homeostasis and preventing oxidative damage. This sophisticated oxidative storage process not only protects cells from oxidative stress and ferroptosis triggered by free iron, but recent studies have also revealed its deep involvement in immune regulation and tissue repair—for example, in regulating macrophage function during kidney injury. Conversely, its dysfunction is closely linked to hereditary hemochromatosis and brain iron accumulation in neurodegenerative diseases.
免疫印迹
WB result of Ferritin Heavy Chain Recombinant Rabbit mAb
Primary antibody: Ferritin Heavy Chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raw 264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Lane 5: mouse heart lysate 20 µg
Lane 6: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDaWB result of Ferritin Heavy Chain Recombinant Rabbit mAb
Primary antibody: Ferritin Heavy Chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat liver lysate 20 µg
Lane 4: rat brain lysate 20 µg
Lane 5: rat kidney lysate 20 µg
Lane 6: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Ferritin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Ferritin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Ferritin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Ferritin heavy chain in mouse & rat tissues.
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