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宿主来源
Mouse抗原名称
COXIV分子别名
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX4; COX4I1细胞定位
MitochondrionAccession
P13073克隆号
S-5528抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Mk阳性样本
HeLa, HepG2, A431, Jurkat, MCF7, LnCap, COS-7纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Hu, Mk IHC-P 1:2000 Hu
COXIV is a key subunit of cytochrome c oxidase (Complex IV), the final enzyme complex in the mitochondrial respiratory chain. This protein consists of approximately 160 amino acids, containing an N-terminal mitochondrial targeting sequence and multiple α-helical transmembrane domains, and is primarily anchored in the inner mitochondrial membrane. COXIV serves dual important functions—structural and regulatory: on one hand, it provides structural support for the core catalytic subunits (COX I and COX II) of Complex IV, assisting in the assembly and stability of the entire enzyme complex; on the other hand, it senses the cellular energy state through an ATP/ADP binding site, thereby allosterically regulating the catalytic activity of Complex IV to match the efficiency of electron transfer in the respiratory chain with ATP synthesis demands. Furthermore, COXIV expression levels vary across different tissues (such as the liver-type COXIV-1 and lung-type COXIV-2 isoforms), and its expression is regulated by transcription factors such as hypoxia-inducible factor HIF-1α, playing a role in cellular responses to hypoxic stress and metabolic reprogramming. At the pathological level, mutations or aberrant expression of COXIV are closely associated with various mitochondrial diseases, including mitochondrial encephalomyopathies and Leigh syndrome. Its dysfunction leads to decreased electron transport chain efficiency and excessive production of reactive oxygen species (ROS), making COXIV also an important potential target in research on ischemia-reperfusion injury, neurodegenerative diseases, and tumor metabolism.
免疫印迹
WB result of COXIV Recombinant Mouse mAb
Primary antibody: COXIV Recombinant Mouse mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: LnCap whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 17 kDaWB result of COXIV Recombinant Mouse mAb
Primary antibody: COXIV Recombinant Mouse mAb at 1/5000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 17 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of COXIV in tumor tissues.
Expression of COXIV in human tissues.
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