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Rabbit抗原名称
RRBP1分子别名
Ribosome-binding protein 1; 180 kDa ribosome receptor homolog (RRp); ES/130-related protein; Ribosome receptor protein; KIAA1398免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
Q9P2E9抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
Caco-2, HepG2, NIH/3T3纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
RRBP1, also known as ribosome-binding protein 1, is a membrane-bound protein located in the endoplasmic reticulum (ER) and is critical for ribosome binding. It plays a role in ER proliferation, secretory pathways, and secretory cell differentiation, as well as mediating ER-microtubule interactions. Alternative splicing of RRBP1 results in protein isoforms with different ribosome-binding affinities and secretory functions. This protein is also implicated in various cancers, such as oral squamous cell carcinoma, cervical squamous cell carcinoma, and colorectal cancer, where its expression is often associated with disease progression and prognosis. Recent studies have shown that RRBP1 regulates blood pressure and potassium homeostasis by modulating intracellular renin trafficking. Additionally, it has been identified as a novel peroxisomal biogenesis factor in human cells.
免疫印迹
WB result of RRBP1 Rabbit pAb
Primary antibody: RRBP1 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 100-140 kDaWB result of RRBP1 Rabbit pAb
Primary antibody: RRBP1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 100-120 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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