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宿主来源
Rabbit抗原名称
CoREST分子别名
REST corepressor 1; KIAA0071; RCOR; RCOR1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q9UKL0克隆号
S-4401-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HL-60, A-673, MOLT-4, Jurkat, HeLa, HT-29, HEK-293, A20, C2C12, PC-12, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:250 Hu, Ms, Rt
The CoREST protein (primarily encoded by the RCOR1 gene, also known as CoREST1) was initially identified as a key corepressor of the transcriptional repressor REST (RE1-Silencing Transcription Factor), functioning by binding to REST to silence neuron-specific gene expression in non-neuronal cells, thereby playing an important role in maintaining cellular identity. Structurally, the CoREST protein contains two SANT domains and an ELM2 domain, which serve as the fundamental basis for its role as a molecular scaffold in recruiting histone-modifying enzymes. Its most distinctive function is to act as the core scaffold of the LSD1-CoREST-HDAC complex, simultaneously binding the histone demethylase LSD1 and the histone deacetylases HDAC1/2, thereby integrating two distinct epigenetic modifying enzymes into a single complex to coordinately regulate chromatin status and gene transcription. Notably, the human genome contains three CoREST family members (CoREST1, CoREST2, and CoREST3), which exhibit differences in their binding affinities for LSD1 and HDAC as well as their transcriptional repressive activities—for instance, CoREST2 binds more weakly to HDAC1/2 due to specific amino acid variations, endowing different complexes with the capacity for fine-tuned regulation of gene expression. Furthermore, recent studies have revealed that CoREST proteins function far beyond the scope of "REST corepressors," as they can independently regulate biological processes such as neural development in a REST-independent manner and play important roles in various diseases.
免疫印迹
WB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: A-673 whole cell lysate 20 µg
Lane 3: MOLT-4 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: HT-29 whole cell lysate 20 µg
Lane 7: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDaWB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDaWB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDaWB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CoREST in tumor tissues.
Expression of CoREST in human tissues.
Expression of CoREST in mouse & rat tissues.
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