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宿主来源
Mouse抗原名称
ALDOA分子别名
Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDA; Aldolase A细胞定位
CytoplasmAccession
P04075抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, HepG2, Jurkat, NIH/3T3, 4T1, mouse liver, rat liver浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Hu, Ms, Rt IHC-P 1:500-1:2000 Hu, Ms, Rt ICC 1:200-1:800 Hu, Ms, Rt
Aldolase A, also known as fructose-bisphosphate aldolase A or ALDOA, is a crucial glycolytic enzyme primarily expressed in skeletal muscle and erythrocytes that catalyzes the reversible cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P), thereby playing a pivotal role in both glycolysis and gluconeogenesis. As a homotetrameric protein belonging to the class I aldolase family, it forms a Schiff base intermediate with its substrate via a specific lysine residue in the active site, distinguishing it mechanistically from class II aldolases that rely on metal ions. Beyond its canonical metabolic function, Aldolase A has been identified as a moonlighting protein involved in various non-glycolytic processes, including actin cytoskeleton organization, cell motility, and mRNA stability, and its dysregulation or overexpression is frequently associated with several pathological conditions, particularly various types of cancer where it supports the heightened energy demands of proliferating tumor cells through the Warburg effect, making it a potential biomarker and therapeutic target in oncology and metabolic disorders.
免疫印迹
WB result of ALDOA Mouse mAb
Primary antibody: ALDOA Mouse mAb at 1/20000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDaWB result of ALDOA Mouse mAb
Primary antibody: ALDOA Mouse mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 4T1 whole cell lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 38-39 kDaWB result of ALDOA Mouse mAb
Primary antibody: ALDOA Mouse mAb at 1/20000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALDOA in tumor tissues.
Expression of ALDOA in human tissues.
Expression of ALDOA in mouse & rat tissues.
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