Histone H2A.X Recombinant Rabbit mAb (S-1772-179)

Histone H2AX,H2a/x,Histone H2A.X,H2AFX,H2AX

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6023

规格

25μl1ml100μl

数量

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产品规格

宿主来源

Rabbit
抗原名称

Histone H2A.X
分子别名

Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P16104
克隆号

S-1772-179
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, MCF7, HepG2, A431, NIH/3T3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP ,
IHC-P ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:5000 Hu, Ms
IHC-P 1:2000 Hu, Ms, Rt
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:5000	Hu, Ms
IHC-P	1:2000	Hu, Ms, Rt
ChIP	1:20-1:50	Hu

背景介绍

Histone H2A.X is a histone variant found in almost all eukaryotes that plays a crucial role in maintaining genome stability. It is highly conserved across species and has a unique C-terminal tail containing an SQ motif, which is phosphorylated by kinases like ATM and ATR in response to DNA double-strand breaks (DSBs) to form γ-H2A.X. This phosphorylation event is a very early and highly conserved response to DNA damage, occurring within minutes in mammals and extending over large chromatin regions around the damage site. γ-H2A.X acts as a major signal to recruit DNA repair factors to the damaged site, facilitating efficient repair and preserving genomic integrity. Deficiencies in H2A.X or its phosphorylation can lead to genomic instability, increased sensitivity to DNA-damaging agents, and a higher risk of tumor formation. Additionally, H2A.X is involved in various other cellular processes, including the regulation of gene expression, immune response, and the establishment of cell fate.

免疫印迹
- WB result of Histone H2A.X Recombinant Rabbit mAb
  Primary antibody: Histone H2A.X Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
- WB result of Histone H2A.X Recombinant Rabbit mAb
  Primary antibody: Histone H2A.X Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-Histone H2A.X antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H2A.X antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-Histone H2A.X antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H2A.X antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
- Chromatin immunoprecipitation (ChIP) was
  performed on HeLa cells cross - linked with 1% formaldehyde
  for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used Histone H2A.X Recombinant Rabbit
  mAb (S-1772-179) and Rabbit mAb IgG Isotype Control
  (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples
  were washed, eluted and cross-links
  reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, AFM, MYOD1, SAT-α and SAT-2 in Histone H2A.X Recombinant Rabbit mAb
  (S-1772-179)-immunoprecipitated sample.
组织表达图谱
- Expression of Histone H2A.X in tumor tissue.
- Expression of Histone H2A.X in human tissue.
- Expression of Histone H2A.X in mouse & rat tissue.